《植物生理学报》 2010, 46(11): 1109-1113

植物种质超低温保存遗传稳定性的研究进展

邵丽, 王子成*

河南大学生命科学学院, 河南开封475004

通信作者：王子成；E-mail: wzc@henu.edu.cn

摘　要：

超低温保存被认为是种质长期保存最有效的方法, 其中生物材料低温保存的遗传稳定性是植物种质资源保存中最受 关注的问题之一。本文对近年来超低温保存后植物材料的遗传稳定性及变异的研究情况进行了介绍, 涉及表型性状分析、 基因组遗传稳定性、表观遗传变化及超低温保存的筛选效应等, 为进一步研究超低温保存的应用提供参考。

关键词：超低温保存; 遗传稳定性; 植物; 脱毒

收稿：2010-08-03   修定：2010-09-15

资助：国家自然科学基金项目(30900973)。

Review of Genetic Stability Research for Plant Germplasm Cryopreservation

SHAO Li, WANG Zi-Cheng*

School of Life Science, Henan University, Kaifeng, Henan 475004, China

Corresponding author: WANG Zi-Cheng; E-mail: wzc@henu.edu.cn

Abstract:

Cryopreservation is considered as the most efficient method of long term plant germplasm conservation, and plant heredity stability of biological materials in low-temperature preservation has become one of the questions which are most worth considering in plant germplasm conservation. In the paper, we reviewed genetic stability and variation of plant materials after cryopreservation in recent years, and gave advice for further study on cryopreservation application, involving phenotypic traits analysis, genome genetic fidelity, epigenetic changes and the screening effect of cryopreservation, etc.

Key words: cryopreservation; genetic stability; plant; virus elimination